Plasmid

Part:BBa_K3887010:Design

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)


pTarget-ΔTnaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 150
    Illegal BamHI site found at 1347
    Illegal XhoI site found at 126
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1410


Design Notes

We use this plasmid changing its sgRNA sequence to knock down the gene TnaA in E.coli by CRISPR-Cas9


Source

From Company Bluepha lab

References