Plasmid
Part:BBa_K3887010:Design
Designed by: Ran Wang Group: iGEM21_BJU_China (2021-10-01)
pTarget-ΔTnaA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1335
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 150
Illegal BamHI site found at 1347
Illegal XhoI site found at 126 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1410
Design Notes
We use this plasmid changing its sgRNA sequence to knock down the gene TnaA in E.coli by CRISPR-Cas9
Source
From Company Bluepha lab